Name: Sam68 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, FC, ELISA
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HeLa cells were subjected to SDS PAGE followed by western blot with 10222-1-AP(KHDRBS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Sam68 Antibody IHC human breast cancer tissue 10222-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10222-1-AP(KHDRBS1 antibody) at dilution of 1:50 (under 10x lens)

IP Result of anti-Sam68 (IP:10222-1-AP, 4ug; Detection:10222-1-AP 1:1000) with HeLa cells lysate 1200ug.

Sam68 Antibody IF mouse brain tissue 10222-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed mouse brain tissue using 10222-1-AP(Sam68 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Sam68 Antibody FC MCF-7 cells 10222-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug Sam68 antibody (10222-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:

human, mouse, rat

Positive WB detected in:

HeLa cells, HEK-293 cells, mouse brain tissue, NIH/3T3 cells

Positive IP detected in:

HeLa cells

Positive IHC detected in:

human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

mouse brain tissue

Positive FC detected in:

MCF-7 cells

Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:20-1:200

IF : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

Sam68 fusion protein Ag0235

Full name:

KH domain containing, RNA binding, signal transduction associated 1

Calculated molecular weight:

48 kDa

Observed molecular weight:

68 kDa

GenBank accession number:

BC000717

Gene ID (NCBI):

10657

Gene symbol

KHDRBS1

Synonyms

KHDRBS1, p62, p68, Sam68

Background

KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. KHDRBS1 has three isoforms with calculated MW of 44-48kd. For isoform with a calculated MW of 48.2 kD, it migrated as a 68kD protein on SDS-PAGE gels[PMID: 10564820]. This ia a rabbit polyclonal antibody raised against residues near the N terminus of human KHDRBS1.

Showing 1- [2] of [2] Publications

Author:		Neumann Manuela M
Pubmed ID:		22842875
Journal:		Acta Neuropathol
Title:		Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.
Application:		IHC

Species:		human

Author:		Cira Dansokho
Pubmed ID:		26912648
Journal:		Brain
Title:		Regulatory T cells delay disease progression in Alzheimer-like pathology.
Application:		IHC

Species:		mouse

WB Figures

Sam68 Antibody WB HEK-293 cells 10222-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP(KHDRBS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10222-1-AP(KHDRBS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10222-1-AP(KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Sam68 Antibody WB mouse brain tissue 10222-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10222-1-AP(KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Sam68 Antibody WB NIH/3T3 cells 10222-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP(KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IHC Figures