Human IL-1 alpha ELISA Kit0 Publications

Catalog number: KE00123

規格

單價

96 T

¥2600

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Overview


Product name:
Human IL-1 alpha ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatants

Assay type:
Sandwich

Sensitivity:
0.7 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Cell culture supernatants 101% 89%-110%
Human plasma 93% 74%-116%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 109.5 6.3 5.7
2 20 290.6 24.5 8.4
3 20 639.4 60.2 9.4

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 128.6 5.0 3.9
2 24 335.7 21.0 6.3
3 24 688.6 62.2 9.0

Product overview:
KE00123 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1 alpha ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 alpha. The assay recognizes human IL-1 alpha. An antibody specific for IL-1 alpha has been pre-coated onto the microwells. The IL-1 alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1 alpha is added to detect the captured IL-1 alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
Hematopoietin 1, IL 1A, IL1, IL1 ALPHA, IL-1 alpha, IL1- alpha, IL1A, IL-1alpha, IL1F1, Interleukin 1 alpha, interleukin 1, alpha
Background

Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 alpha is a cytokine and a potent mediator of body’s response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer’s disease and tumor growth. IL-1 alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.


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